genepool

Next Generation Sequencing and Bioinformatics
at the University of Edinburgh


Comparison of Installed Sequencing Technologies in the GenePool

This matrix illustrates the different outputs of the sequencing technologies available at the GenePool, and also indicates the kinds of research applications to which they are suited. The global activity in next generation sequencing is such that new applications are being devised frequently. The GenePool strives to offer these as they mature.

See the technology-specific pages linked from the menu above for more details.

Platform:

Features:

ABI3730 (Sanger dideoxy) Roche Titanium
(454)
Illumina GAII
(SOLEXA)
Illumina GAIIx (SOLEXA)

Data per instrument run (single-end)

0.5 Mb

400 Mb

5000 Mb

>7000 Mb

Reads per instrument run (single-end)

96

800,000

>70,000,000

>100,000,000

Length of reads

up to 900 bases

up to 500 bases

up to 75 bases

75 bases and above

Typical cost, per base of raw data

£0.005

£0.00002

£0.000002

£0.000001

Time for one instrument run

4 hr

12 hr

72-144 hr

72-144 hr

Can do part runs:

YES
up to 16 lanes

YES
up to 8 lanes

YES
up to 8 lanes

Paired-end sequencing (length of read pairs)

YES (600 b)

YES (200 b)

YES (50-75 b)

YES (up to 75 b)

Applications

De novo Genome sequencing:

(YES)

YES

YES

YES

Genome resequencing

YES

YES

YES

De novo Transcriptome sequencing

YES (from clones)

YES

YES

YES

Global transcript abundance measurement (deep SAGE and RNA-Seq)

(YES)

YES

YES

Chromatin immunoprecipitation (ChIP) and RNA-precipitation (RNAIP) sequencing

YES

YES

YES

Small RNA (including miRNA) sequencing

YES

YES

Population genetics, phylogenetics and DNA barcoding from individual specimens

YES

 

Population genetics, phylogenetics and DNA barcoding from communities

YES

YES

YES

End-sequencing of large-insert clones.

YES

 

Genome or large-insert clone de novo sequencing

YES

YES

YES

YES

Genome or large-insert clone resequencing

YES

YES

YES

Metagenomic sequencing

YES

 

Contact us for more information and a quotation genepool@ed.ac.uk

 



The GenePool has core support from: GenePool admin site
edinburgh
The University of Edinburgh
School of Biological Sciences
The Darwin Trust
of Edinburgh
darwin
mrc
nerc
bbsrc
sulsa

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This is version 2.2 of the GenePool website (July 2009).