genepool

Next Generation Sequencing and Bioinformatics
at the University of Edinburgh


Methods used by the GenePool

Sanger Sequencing: Fosmid End Sequencing Protocol.

                          1 sample        1 plate
   BigDye                 4 µl            424 µl
   Primer (10 µm)         2 µl            212 µl
   5x Sequencing Buffer   4 µl            424 µl 
   dH2O                   5 µl            530 µl

198 µl in each well of a strip and aliquot 15 µl to each well.

Template 5 µl in each well

Place on FAST PCR machine.

   96°C    1 minute
   95°C    10 seconds
   50°C    5 seconds
   60°C    1 minutes 15 seconds

99 cycles, hold at 4°C


The GenePool has core support from: GenePool admin site
edinburgh
The University of Edinburgh
School of Biological Sciences
The Darwin Trust
of Edinburgh
darwin
mrc
nerc
bbsrc
sulsa

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This is version 2.2 of the GenePool website (July 2009).