![]() |
Next Generation Sequencing and Bioinformatics |
Methods used by the GenePool Sanger Sequencing: PCR Reaction volumes per sample (20 µl) per plate 2 plates 3 plates 10X buffer (1.5mM MgCl2) 2 µl 200 µl 400 µl 600 µl Primer F (10pmol/ µl) 0.4 µl 40 µl 80 µl 120 µl Primer R (10pmol/ µl) 0.4 µl 40 µl 80 µl 120 µl dntps (2mM) 2 µl 200 µl 400 µl 600 µl Taq (5 U/ µl) 0.2 µl 15 µl 30 µl 45 µl dH2O 13 µl 1305 µl 2610 µl 3915 µl DNA template 2 µl in each Cycle: 94°C 3mins 94°C 15 sec} 55°C 40sec } - 35 cycles 72°C 3 min } 72°C 10min hold at 4°C 1. Make up a master mix and dispense 225 µl into each well of an 8-strip tube. |
The GenePool has core support from: | GenePool admin site | |||
![]() |
The University of Edinburgh School of Biological Sciences |
The Darwin Trust of Edinburgh |
![]() |
|
![]() |
![]() |
![]() |
![]() |