genepool

Next Generation Sequencing and Bioinformatics
at the University of Edinburgh


Sanger Sequencing at The GenePool

What we offer | Who we offer it to | Costs | FAQ | Sample submission

The GenePool operates two ABI 3730 capillary sequencing instruments, and can carry out a range of projects from single sequencing reads to medium-scale projects involving tens of thousands of reads.


What we offer

Analysis of sequenced samples

The user can deliver samples that have already been sequenced with BigDye reagents. We perform clean-up and capillary analysis on the ABI3730 (any number of samples).

Full sequencing reaction

The user can deliver clean DNA and sequencing primer. We perform the BigDye sequencing, clean-up and capillary analysis on the ABI3730 (any number of samples).

Larger sequencing projects

The facility is fully equipped to perform larger-scale Sanger sequencing projects. These projects might include expressed sequence tag (EST) surveys, population genetics surveys, sequencing of large insert clones (cosmids, fosmids and BACs), metagenomic surveys, and genome sequencing and resequencing. These projects might typically involve 1000-10000 samples.

These larger projects are processed in batches of 96 (i.e. in 96-well plates). We can perform shotgun subcloning, and insert DNA preparation. We also offer bioinformatics support for assembly and annotation.

Genotyping with microsatellites

We can analyse your PCR-amplified microsatellite or AFLP samples (either 48 or 96 samples at a time) on the ABI3730 instruments. We can either return the data to you, or assist by giving you access to GeneMapper tools.

For University of Edinburgh researchers we can also offer collaboration in performing the microsatellite analyses for you (from DNA extraction, through PCR and multiplexing, to analysis and allele calling). Please contact the GenePool well in advance of grant submission deadlines to discuss this with us.


Some of the protocols we use are available here.


Who we offer Sanger sequencing to

We offer Sanger sequencing to

  • Edinburgh University researchers
  • Researchers in Institutes and other research centres in the Edinburgh area
  • NERC science through the NERC Biomolecular Analysis Facility (NBAF). Researchers from throughout the UK whose science falls within the NERC science area can apply for access to our Sanger sequencing facility.

We do not usually offer access to our Sanger technology to groups outside these constituencies.

Please contact us to discuss your Sanger sequencing or genotyping needs.


The GenePool has core support from: GenePool admin site
edinburgh
The University of Edinburgh
School of Biological Sciences
The Darwin Trust
of Edinburgh
darwin
mrc
nerc
bbsrc
sulsa

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This is version 2.2 of the GenePool website (July 2009)