Next Generation Sequencing and Bioinformatics
Sanger Sequencing at The GenePool
The GenePool operates two ABI 3730 capillary sequencing instruments, and can carry out a range of projects from single sequencing reads to medium-scale projects involving tens of thousands of reads.
Analysis of sequenced samples
The user can deliver samples that have already been sequenced with BigDye reagents. We perform clean-up and capillary analysis on the ABI3730 (any number of samples).
Full sequencing reaction
The user can deliver clean DNA and sequencing primer. We perform the BigDye sequencing, clean-up and capillary analysis on the ABI3730 (any number of samples).
Larger sequencing projects
The facility is fully equipped to perform larger-scale Sanger sequencing projects. These projects might include expressed sequence tag (EST) surveys, population genetics surveys, sequencing of large insert clones (cosmids, fosmids and BACs), metagenomic surveys, and genome sequencing and resequencing. These projects might typically involve 1000-10000 samples.
These larger projects are processed in batches of 96 (i.e. in 96-well plates). We can perform shotgun subcloning, and insert DNA preparation. We also offer bioinformatics support for assembly and annotation.
Genotyping with microsatellites
We can analyse your PCR-amplified microsatellite or AFLP samples (either 48 or 96 samples at a time) on the ABI3730 instruments. We can either return the data to you, or assist by giving you access to GeneMapper tools.
Some of the protocols we use are available here.
We offer Sanger sequencing to
We do not usually offer access to our Sanger technology to groups outside these constituencies.
Please contact us to discuss your Sanger sequencing or genotyping needs.
|The GenePool has core support from:||GenePool admin site|
|The University of Edinburgh
School of Biological Sciences
The Darwin Trust