genepool

Next Generation Sequencing and Bioinformatics
at the University of Edinburgh


Sanger Sequencing     Methods used by the GenePool

Here you will find precis of some of the methods used by the GenePool in processing and analysing samples on our ABI 3730 instruments. They work for us...


Preparing samples for sequencing

Subcloning large-insert clones for shotgun sequencing

  • Fosmid Prep Protocol
  • Nebulising Protocol
  • Blunt Ending Protocol
  • Dephosphorylation Protocol
  • TOPO Cloning Protocol
  • Electroporation Protocol
  • Colony Picking Protocol

Sequencing protocols

Genotyping

  • Genemapper Info

Please contact us to discuss your Sanger sequencing or genotyping needs, and for current pricing.


The GenePool has core support from: GenePool admin site
edinburgh
The University of Edinburgh
School of Biological Sciences
The Darwin Trust
of Edinburgh
darwin
mrc
nerc
bbsrc
sulsa

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This is version 2.2 of the GenePool website (July 2009).